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題名 | A Model for Investigating Effect of Shock Waves on Intracellular Calcium Mobilization=建立研究震波對細胞內鈣離子移動之影響的模式 |
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作者 | 陳偉權; 簡崇仁; 李瀛輝; 歐秀中; 黃榮慶; 曾清俊; Chen, Wei-chuan; Jan, Chung-ren; Lee, Ying-huei; Ou, Hsio-chung; Huang, Jong-khing; Tseng, Ching-jiunn; |
期刊 | 中華醫學雜誌 |
出版日期 | 19961100 |
卷期 | 58:5 1996.11[民85.11] |
頁次 | 頁341-347 |
分類號 | 416.274 |
語文 | eng |
關鍵詞 | 鈣離子攝影; MDCK腎細胞株; 碎石震波; Calcium imaging; Fura-2; MDCK cells; Shock waves; |
中文摘要 | 背景 體外震波碎石術對尿路結石的治療,已成為一種幾乎是非侵犯 性的標準治療模式;然而,它仍有一些不適的生物效應。我們已建立一個 Madin-Darby氏狗腎小管 (MDCK) 細胞株,並利用鈣離子攝影的實驗模式,來 研究此類細胞內游離鈣離子濃度變化的情形。 方法 我們利用數位化影像分析,使用fura-2螢光染劑,來觀察單一MDCK 細胞在靜止及激發狀態時,胞內游離鈣離子濃度變化的情形。我們利用腺核甘 三磷酸 (ATP),緩動素 (bradykinin) 及thapsigargin三種藥劑來升高胞內鈣離子 濃度。 結果 ATP,bradykinin及thapsigargin均使胞內鈣離子濃度短暫及強烈的增加。 結論 我們已建立一個能研究碎石震波對於單一腎細胞影響的模式。運用此模 式,我們能了解碎石震波如何影響胞內鈣離子濃度的調節。另外,也讓我們能 了解鈣離子阻斷劑,如nifedipine,verapamil,是否能利用阻斷鈣離子流入胞內, 來保護腎細胞免於碎石震波的破壞。 |
英文摘要 | Background. Shock wave lithotripsy (SWL) has become a non-invasive standard treatment for urolithiasis; however, it has some unwanted bioeffects. A cell model using calcium (Ca) imaging of cultured Madin-Darby canine kidney (MDCK) cells has been established in this laboratory to study the effects of shock waves on intracellular free Ca mobilization of renal tubular cells. Methods. Digital video imaging of fura-2 fluorescence was used to measure both resting and stimulated intracellular free Ca concentrations in single cultured MDCK cells. Pharmacological agents including adenosine-5'-trisphosphate (ATP), bradykinin and thapsigargin, were used as Ca mobilizing agents. Results. ATP, bradykinin and thapsigargin all elicited a robust transient increase in intracellular Ca concentration. Conclusions. A cell model was established to investigate the effect of shock waves on single kidney cells. This provided an opportunity to determine how shock waves affect the regulation of intracellular Ca concentrations in kidney cells; in addition, it allows investigation, for the first time at the single cell level, of whether blocking Ca entry in kidney cells plays any role in the mechanism by which some Ca channel blockers, e.g. nifedipine and verapamil, protect patients of urolithiasis from shock wave-induced renal damage. |
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