頁籤選單縮合
題名 | The Comparison between Ischemic Precondition (IP) and Intermittent Reperfusion (IR) Effects on Tibial Microcirculation= |
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作者 | 杜元坤; 許汝寧; 葉文凌; 顏政佑; 王坤全; 翁文能; Tu, Yuan-kun; Hui, Yu-ling; Yeh, Wen-lin; Yen, Chen-yo; Wang, Kung-chung; Ueng, Steve Wen-neng; |
期刊 | 中華民國骨科醫學會雜誌 |
出版日期 | 20000300 |
卷期 | 17:1 2000.03[民89.03] |
頁次 | 頁1-10 |
分類號 | 416.26 |
語文 | eng |
關鍵詞 | Ischemic precondition; Intermittent reperfusion; Reperfusion injury; |
英文摘要 | Purpose: In the skeletal microcirculation, is chemic precondition (IP) has been proved to attenuate the ischemic/reperfusion injury. However, is chemic precondition is impossible for application one post-traumatic replantation. In the study, we investigated the effect of intermittent reperfusion (IR) prior to reperfusion that is similar to our clinical practice of skeletal replantation, and compared the effect of IP and IR vascular endothelium. Materials and Methods: Forty adult New Zealand rabbits were anesthetized, and their nutrient arteries of tibia were identified. 40 rabbits were evenly divided into 4 groups. Group 1: control, 4 hours ischemia without IR or IP; Group 2: IP by 3 cycles of pedicle clamping and reperfusion for 15 minutes, prior to 4 hours of ischemia; Group 3: IR by 3 cycles of intermittent reperfusion, after 4 hours of ischemia; and Group 4: The tibia were treated with IP and IR together. We put the tibia into bone chamber after cannulation, and then compared the effect of vasospasm by norepinephrine dose-response curve (NEDRC). Acetylcholine and L-NMMA were also perfused and the NEDRC recorded. Results: The NEDRC data in group 1 was set to be 100%. Our results showed that IP significantly attenuated NEDRC in group 2 (49.2%, p<0.01). IR alone showed only mild decreases in NEDRC (87.1%, p>0.05). Combined IP and IR attenuated vasospasm most effectively (36.8%, p<0.01). Group 4 tibia showed the lowest NEDRC under Acetylcholine perfusion. L-NMMA perfused data revealed that IR combined with IP could reduce the most of vasospasm caused by ischemia. Conclusions: Based one our study, IP or IP combined with IR are useful techniques for attenuating ischemic/reperfusion injury. IR alone is not effective in reducing vasospasm after ischemia. |
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