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題 名 | Effects of Gas Composition on the Cell Growth and 戹-DOPA Production in the Suspension Culture of Stizolobium hassjoo=氣相組成對藜豆細胞懸浮培養之細胞生長及戹-DOPA生產之影響 |
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作 者 | 黃世佑; 胡晉嘉; 王自存; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷 期 | 32:1 2001.01[民90.01] |
頁 次 | 頁1-11 |
分類號 | 434.28 |
關鍵詞 | 氣相組成; 藜豆細胞; 細胞生長; 戹-dopa production; Gaseous composition; Oxygen; Carbon dioxide; Ethylene; Scale-up; |
語 文 | 英文(English) |
中文摘要 | 為探討植物細胞培養系統中代謝氣體(O₂,CO₂,C₂H₄等)對細胞生長及二次代謝產物生產之影響,使用附斜檔板之錐形瓶及使用不同透氣性之瓶塞以改變瓶中之Po₂及氧氣傳遞速率。使用連體錐形瓶以探討不同CO₂及C₂H₄濃度對細胞生長及二次代謝產物生產之影響。高Po₂有利於L-dopa之生成量。溶氧及KLa為影響L-dopa生成量之重要因素,低乙烯含量有利於細胞生長及L-dopa之生產。過高之CO₂阻抑細胞之生長及L-dopa之生產。植物細胞培養系之擴大規模策略應著重控制培養中溶氧濃度(35%飽和),維持低C₂H₄濃度(0.02-0.2 ppm)及低CO₂ 濃度(Pco₂ <5%)。本研究提供植物細胞培養系最佳氣相組成之範圍。 |
英文摘要 | Effects of gaseous metabolites, O₂,CO₂ and C₂H₄ in plant cell culture system on cell growth and secondary metabolite formation were investigated. Po₂ in the shake flask was regulated by employing different closures, different volumes of medium and shaking rates of the flasks. A creased Erlenmeyer flask promoted remarkable the oxygen transfer rate thus favored L-dopa production. A two-tier culture vessels were employed to elucidate the effects of CO₂ and C₂H₄ on culture performances. Within the concentration range of oxygen studied (below 21 %), the higher the Po₂ the higher the in vivo L-dopa content although the cell mass concentration was not affected profoundly by Po₂. It was found that both the dissolved oxygen and kLa of the culture medium were the important factors for L-dopa production. The concentration of C₂H₄ was closely related to the L-dopa production. The low C₂H₄ concentration favored the cell growth and L-dopa production. It was found that the CO₂ was indispensable to plant cell metabolism. However, accumulation of CO₂ in the culture system inhibited the cell growth and L-dopa formation. The strategy to facilitate the production of L-dopa in a bioreactor was to control the optimum D.O. concentration thus alleviate the oxygen limitation in the medium. The lower the Pc₂H₄, the higher the L-dopa productivity. The removal of CO₂ by 20% KMnO₄ solution enhanced the L-dopa synthesis. The results obtained in this work are thought to be a guideline for maintaining an optimum gaseous composition in plant cell culture reactors. |
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