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題名 | Coactivator TIF1β Interacts with Transcription Factor C/EBPβ and Glucocorticoid Receptor to Induce α1-Acid Glycoprotein Gene Expression= |
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作者 | Chang,Ching-jin; Chen,Ya-ling; Lee,Sheng-chung; |
期刊 | 中央研究院生物化學研究所論文集 |
出版日期 | 19980000 |
卷期 | 24 1998[民87.] |
頁次 | 頁1-8 |
分類號 | 361.4 |
語文 | eng |
關鍵詞 | TIF1β; C/EBPβ; α1-acid glycoprotein gene; Agp gene; |
英文摘要 | The transcription of the α1-acid glycoprotein gene is induced by inflammatory cytokines and glucocorticoids. C/EBP β is a major transcription factor involved in the induction of the agp gene by some cytokines. In this report, we have identified a novel transcriptional intermediary factor, TIF1 β. which could enhance the transcription of the agp gene by the glucocorticoid receptor (GR) and C/EBP β. TIFIp belongs to a subgroup of RING (really interesting new gene) finger proteins that contain a RING finger preceding two B box-type fingers and a putative coiled-coil domain (RBCC domain). Immunoprecipitation experiments showed that the interaction between GR and TIF1 β is ligand independent. The overexpression of the TIF1 β gene enhances GR-regulated expression in a ligand- and glucocorticoid-responsive element (GRE)-dependent manner. TIF1 β can also augment C/EEP β -mediated activity on wild-type and GRE-mutated agp genes, but this augmentation is diminished when all three C/EBP β -binding elements are mutated. Functional and biochemical characterizations indicated that the bZIP domain of C/EBPp and the RBCC domain, plant homeodomain finger, and brnmodomain of TIF1 β are crucial for the interactions of these proteins. Taken together, these results suggest that TIF1 β serves as a converging mediator of signal transduction pathways of glucocorticoids and some inflammatory cytokines. |
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