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題名 | Spatial and Temporal Analysis of Nociception-related Spinal Cord Matrix Metalloproteinase Expression in a Murine Neuropathic Pain Model= |
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作者 | Liou, Jiin-tarng; Sum, Daniel Ching-wah; Liu, Fu-chao; Mao, Chih-chieh; Lai, Ying-shu; Day, Yuan-ji; |
期刊 | Journal of the Chinese Medical Association |
出版日期 | 20130400 |
卷期 | 76:4 2013.04[民102.04] |
頁次 | 頁201-210 |
分類號 | 415.9 |
語文 | eng |
關鍵詞 | Animal model; Chronic pain; Matrix metalloproteinases; Neuropathic pain; Spinal cord; |
英文摘要 | Background: Although the mammalian central nervous system contains numerous matrix metalloproteinases (MMPs), the significance of each MMP relative to nociception remains obscure. Working from the hypothesis that MMPs may be involved in activity-dependent reorganization during neuronal modulation, we explored the role of each MMP following neuropathological injury by establishing MMP expression profiles in a murine model for neuropathic pain. Methods: Sciatic nerves of adult male C57BL/6C mice were partially ligated, and their responses to mechanical and radiant heat stimulations were observed at 1, 3, 7, and 14 days. The expression of several nociception-related MMPs (MMP-2, MMP-9, MMP-12, MMP-17, and MMP24) in the spinal cord was detected by immunohistochemical analysis, Western blotting, and real-time polymerase chain reaction. In addition, the potential of GM6001, a general inhibitor of MMP peritoneal administration, to modulate nociceptive pain responses in a chronic neuropathic pain model in mice was also investigated. Results: MMP-2, 9, 17, and 24, but not MMP-12, were expressed in the murine spinal cord. MMP-9 was constitutively expressed in neurons and microglial cells, immediately upregulated after nerve injury, and returned to baseline levels at day 3. Expression of MMP-2, MMP-17, and MMP-24 gradually increased after nerve injury. Morphologically, MMP-2-positive cells were glial-like cells. MMP-17 and MMP-24 expression was widespread in gray matter, neurons, and microglial cells, and concentrated in the marginal zone of the dorsal horn and in small capillaries. Peritoneal administration of GM6001 resulted in significantly attenuated thermal hyperalgesia and tactile allodynia induced by nerve injury. Conclusion: Expression of several nociception-related MMPs was differentially regulated both temporally and spatially following nerve injury. These results suggest that neuronal remodeling requires concerted expression of particular MMPs in specific temporal and spatial patterns, which may be necessary for neuronal plasticity and/or recovery. |
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