查詢結果分析
來源資料
頁籤選單縮合
題名 | 臺南白玉米自交系RAPD分析的遺傳歧異度與其F[feb5]雜種優勢之相關=The Relationship between RAPD Heterozygosity and Heterosis of Inbred Lines Derived from Tainan-White Maize Populations |
---|---|
作者 | 謝光照; 曾富生; Shieh, Guang-jauh; Thseng, Fu-sheng; |
期刊 | 中華農學會報 |
出版日期 | 20011000 |
卷期 | 2:5 2001.10[民90.10] |
頁次 | 頁402-413 |
分類號 | 434.11 |
語文 | chi |
關鍵詞 | 玉米; 遺傳歧異度; F[feb5]雜種; 籽粒產量; 雜種優勢; Maize; Genetic diversity; F[feb5]hybrids; Grain yield; Heterosis; |
中文摘要 | 本研究之主要目的在探討臺南白玉米自交系RAPD分析的遺傳歧異度與其□雜種之雜種優勢的相關性。試驗以臺南白族群所分離的13個自交系及其半全互交的78個□雜種,及天然混合授粉之臺南白(對照種)為材料,在1996年秋作(9月23日)種植於農試所試驗田進行農藝性狀調查及籽粒產量之評估。並進行13個自交系之RAPD分析。結果顯示所使用的13個臺南白自交系間其農藝性狀及RAPD分析的DNA條帶都有明顯的差異存在。自交系所產生的RAPD多型性條帶經由Jaccard’s公式計算,顯示自交系遺傳距離之變異介於0.421~0.772之間。同時亦發現自交系間Jaccard’s遺傳距離與其相對的□雜種(兩親本之和)之一般組合力(GCA)間無相關存在;Jaccard’s遺傳距離與特殊組合力(SCA)及對照雜種優勢(Hck)間之相關達極顯著,其□值分別為0.125及0.137。因此臺南白玉米自交系間RAPD之遺傳歧異度對□雜種籽粒產量之一般組合力、特殊組合力及對照雜種優勢的大小無法很有效的預測。 |
英文摘要 | The objective of this study was to evaluate the genetic diversity of 13 maize inbred lines, and to determine the relationship between genetic distance of the parental lines and their single cross hybrid heterosis. The randomly amplified polymporphic DNA (RAPD) technique was used. Six of these lines came from the Taichung populations, and the rest of inbred lines derived from other seven different population were available in this study. The agronomic traits of the 78 single crosses by half-diallel cross and their 13 parental lines were evaluated in the fall of 1996 at TARI. One Tainan white open pollinated population was the check. Forty different 10-primers were used to give a total of 646 reproducible amplification products, 547 (84.7%) of them being polymorphic. Genetic diversity was determined using Jaccard’s similarity coefficient. The results of this study showed that the inbred lines had significant difference in phenotypic traits and DNA sequence. The range of genetic distance between parental lines was from 0.421 to 0.772. There was no relationship existed between genetic distance and general combining ability (sum of the parental lines) of grain dry weight. But a significant relationship between the specific combining ability (SCA) and standard heterosis value (Hck) of grain dry weight of hybrid on genetic distance. However their coefficient of determination (□) are low. So that the RAPD genetic diversity isn’t effective to predicted the combining ability and standard heterosis value (Hck) of grain dry weight. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。