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題 名 | 短期與長期葉酸缺乏對大白鼠葉酸營養狀態與肝臟細胞核,粒線體及微粒體之脂質過氧化的影響=The Short- and Long-term Effects of Folate Deficiency on Folate Status and Lipid Peroxidation in Nuclear, Mitochondrial and Microsomal Fractions Isolated from Rat Livers |
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作 者 | 許瑞芬; 許玉卿; | 書刊名 | 中華民國營養學會雜誌 |
卷 期 | 25:3 2000.09[民89.09] |
頁 次 | 頁132-139 |
分類號 | 415.136 |
關鍵詞 | 葉酸缺乏; 細胞核; 粒線體; 微粒體; 脂質過氧化; 肝臟; Folate-deficiency; Liver; Nucleus; Mitochondria; Microsome; Lipid peroxidation; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究以雄性離乳Wistar大白鼠為實驗動物,餵以amino acid-defined 之基礎飼料配方不含葉酸(葉酸缺乏組),或添加葉酸8mg/kg diet使為對照組,為期二或六週。葉酸缺乏組之血葉酸營養指標在二週後即比對照組顯著降低,持續葉酸缺乏六周後血紅素也顯著降低。葉酸缺乏二週後,肝臟葉酸含量降為對照組的20%,六週後降為對照組的4%。肝臟細胞質液與粒線體葉酸含量佔肝臟均質液之葉酸含量的87~90%,肝臟葉酸缺乏主要反應於細胞質與粒線體葉酸含量顯著降低( p <0.05),降低至對照組的3~13%。葉酸缺乏二週時肝臟均質液內生性脂質過氧化傷害(TBARS值:thiobarbituric acid reactive substances)尚無明顯增加,六週後增加為對照組的2.3倍,加入過氧化氫或鐵誘發的TBARS值均比對照組顯著增加。葉酸缺乏二週尚未對肝臟細胞核,粒線體及微粒體造成內生性或外誘性脂質過氧化傷害;但在鐵誘發下細胞核TBARS值顯著增加。葉酸缺乏六週後,微粒體內生性TBARS值顯著增加。外誘性過氧化氫或鐵的處理,均使細胞核,粒線體及微粒體的TBARS值顯著高於對照組。以上結果顯示,隨著體內葉酸消耗的嚴重程度之增加,肝臟之內生性或外誘性脂質過氧化傷害顯著增加;同時造成肝臟細胞核,粒線體及微粒體顯著增加的氧化傷害。 |
英文摘要 | The purpose of this study was to investigate the short- and long-term effects of folate deficiency on the folate status and lipid peroxidation in the nuclear, mitochondrial and microsomal fractions extracted from rat livers. Weaning Wistar male rats were fed an amino acid-defined folate-deficient basal diet (folate deficient group) or a basal diet supplemented with 8 mg folic acid/kg diet (control group) for 2 and 6 weeks. Plasma and red blood cell folate levels in the rats fed folate deficient diets for 2 or 6 weeks were significantly lower than those for the control rats (p<0.05). Hepatic folate status was decreased to 20% of the control levels by 2 weeks of feeding folate deficient diets, and further decreased to 4% of the control levels after 6 weeks of folate deficiency. The inferior hepatic folate status in folate-deficient rats was reflected by the markedly decreased folate concentrations in cytosolic and mitochondrial liver homogenate fractions. The endogenous lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) values in the liver homogenate of rats fed folate-deficient diets for 2 weeks were not different from the control group, yet significantly increased by 2.3 fold after 6 weeks of feeding folate deficient diets as compared to the control group. Lipid peroxidation induced by H2O2 or Fe2+. in liver homogenates from folate-deficient rats was higher than these in the control group. Folate deficiency for 2 weeks did not result in either endogenous or H2O2-induced lipid peroxidation damage in the nuclear, mitochondrial and microsomal fractions, except for nucelar lipid peroxidation induced by Fe2+. A 6-week folate deficiency resulted in a significant increase in endogenous microsomal TBARS values and a significant increase in H2,O2, and Fe2+ -induced lipid peroxidation damage in the nuclear, mitochondri-increase in endogenous or induced-lipid peroxidative damage either in the liver homogenates or in the intracellular organelles such as nuclear, mitochondria or microsome in rats. |
本系統中英文摘要資訊取自各篇刊載內容。