頁籤選單縮合
題名 | Analysis of an SMN Gene-Like DNA Fragment=SMN類似基因的分析 |
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作者 | 鐘育志; 李鴻; 張建國; 李謝秀梅; 李賢雄; 李正淳; 王妙媛; 蔡長海; Jong, Yuh-jyh; Li, Hung; Chang, Jan-gowth; Li Hsieh , Hsiu-mei; Lee, Hsien-hsiung; Lee, Cheng-chun; Wang, Nancy M.; Tsai, Chang-hai; |
期刊 | The Kaohsiung Journal of Medical Sciences |
出版日期 | 20000300 |
卷期 | 16:3 2000.03[民89.03] |
頁次 | 頁112-116 |
分類號 | 417.107 |
語文 | eng |
關鍵詞 | SMN類似基因; Spinal muscular atrophy; Survival motor neuron gene; Pseudogene; |
中文摘要 | 我們利用RT-PCR的方法分析SMN基因的表現,分析其產物偶然發現 一段與SMN基因非常相似的產物,其DNA序列的分析結果顯示有74.6%的核�� 酸同質性,但其中含有幾個停止的密碼,我們又發現此基因片斷在各種組織並無 表現,因此,我們認為它是一個SMN偽基因的片斷。此偽基因片斷並無intron, 而且有些正常人並無此基因。 |
英文摘要 | Part of a survival motor neuron (SMN) gene-like DNA fragment has been identified. This DNA fragment was accidentally isolated from cDNA by RT-PCR using primers specific for the region between exon 3 and 6 of the SMN gene. This fragment was used as a probe to hybridize the mRNA from several tissues, but we have been unable to detect any transcript of this SMN-like gene in these tissues. Thus, we have inferred this SMN gene-like fragment was a genomic product contaminant that was amplified in the reaction. Sequencing analysis of this fragment, which contains several stop codons, revealed a 74.6% nucleotide homology with the SMN gene. From these results, we believe that this DNA fragment is not a mutated form of SMN gene. Rather, it is an SMN-like pseudogene, which is variably present even in normal individuals. |
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