查詢結果分析
相關文獻
- Cytochemical Profiles and Quantitative Analysis of Fiber Types in Trunk Muscle of Tigerperch, Terapon jarbua
- Morphometric and Histochemical Characteristics of Fiber Types in Trunk Muscle of Mullet, Mugil cephalus
- Effects of Zinc Deficiency on Endogenous Antioxidant Enzymes and Lipid Peroxidation in Glomerular Cells of Normal and Five-Sixths Nephrectomized Rats
- Production, Purification and Characterization of an Extracellular Catalase from Penicillium SP. TS-622
- 行道樹淨化空氣污染能力之研究(1)
- 生羊乳之乳過氧化酵素系統及其延長貯存期限之研究
- 邊緣性葉酸缺乏對F344大白鼠葉酸營養狀態、肝臟抗氧化能力及脂質過氧化傷害之影響
- 簡介第二型環氧化酵素抑制劑
- 抗壞血酸處理對楊桃(Averrhoa carambola, L.)多酚氧化酵素活性之影響
- 環氧化酵素與非類固醇抗發炎藥物的新進展
頁籤選單縮合
題名 | Cytochemical Profiles and Quantitative Analysis of Fiber Types in Trunk Muscle of Tigerperch, Terapon jarbua=花身雞魚(Terapon jarbua)軀幹肌之肌纖維分類:細胞化學圖譜及定量分析之研究 |
---|---|
作者 | 陳雪芬; 黃寶貴; | 書刊名 | 動物研究學刊 |
卷期 | 39:1 2000.01[民89.01] |
頁次 | 頁28-37 |
分類號 | 387.1017 |
關鍵詞 | 形態測定學; 氧化酵素; 灰度值; 肌纖維分類; Morphometry; mATPase; Oxidative enzyme; Gray-level; Typing fiber; |
語文 | 英文(English) |
中文摘要 | 本研究係以連續組織切片之細胞化學圖譜及影像分析技術對花身雞魚(Terapon jarbua)軀幹肌之肌纖維做形態學上的分類。首先以mATPase的反應做為肌纖維型鑑別之依據,再以SDH、NADH-TR及LDH之反應探討肌纖維之代謝型式,最後再檢定肌纖維中肝糖及脂質的含量以了解能量利用之來源。根據上述組織化學染色反應之結果,可將軀幹肌區分為白肌、紅肌及粉紅肌三種,又根據肌纖維的面積大小及氧化醏素反應的活性(以灰度值做定量分析),發現每一種肌肉是由一種以上不同類型之肌纖維所構成。白肌主要是由一種肌纖維所構成,此肌纖維相當於哺乳類之type llb fibers並具有較大之橫切面積;紅肌則是由大量的type llc fibers 並具有較大之橫切面積;紅肌則是由大量的type llc fibers(面積最小)及少量散布於llc fibers 周圍之type I fibers (具中等面積)所構成。此外,由上述反應結果發現,粉紅肌則是軀幹肌中肌纖堆組成最複雜的,其至少是由type l、llb及llc三種肌纖維以鑲嵌狀(mosaic)排列方式所構成。 |
英文摘要 | Fiber types in trunk muscle of the tigerperch; Terapon jarbua. were classified by cytochemical profiles and image analysis techniques. Serial cross-sections of trunk muscle were examined by evaluating myosin ATPase(mATPase) to categorize the muscle fiber types, by measuring the oxidative enzyme activities of SDH, NADH-TR, and LDH to identify the metabolic patterns, and by determining the glycogen and lipid contents to specify the energy sources. The study revealed 3 muscle patterns (white, red, and pink muscles) distinguished by their enzyme activities. The different fiber types differed in their m ATPase activities after preincubation in different pH solutions. Oxidative enzyme activities were quantitatively analyzed and correlated to their gray-level and fiber size. In white muscle only 1 histochemical fiber type (resembling type llb fiber in mammals, with a large cross-sectional area, CSA) could be identified. Red muscle consisted of abundant type llc fibers (with a smaller CSA) and a few type l fibers (with a medium CSA) sparsely interposed among llc fibers. Fibers in a mosaic arrangement of pink muscle, with different histochemical reactions and of various sizes, were classified as type l, llb, and llc fibers. Type llb fiber had larger cross-sectional areas (2969.30 ±237.27~3045.66±1088.87 um2), type llc had smaller (318.77±105.02~353.20±122.18 um2), and type l had medium areas (708.65±142.62~1192.09±308.28 um2). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。